Prokaryotic Community Analysis of a Hyperalkaline Spring in the Philippines Using 16S rRNA Gene Clone Library Construction

Baculi, Ronan Q.; Lantican, Nacita B.; de los Reyes, Francis L. III; Raymundo, Asuncion K.

dc.contributor.author	Baculi, Ronan Q.
dc.contributor.author	Lantican, Nacita B.
dc.contributor.author	de los Reyes, Francis L. III
dc.contributor.author	Raymundo, Asuncion K.
dc.date.accessioned	2020-02-13T07:50:17Z
dc.date.available	2020-02-13T07:50:17Z
dc.date.issued	2015-06
dc.identifier.citation	Philippine Journal of Science 144 (1): 1-12, June 2015	en_US
dc.identifier.uri	http://dspace.upb.edu.ph/jspui/handle/123456789/75
dc.description	This work was part of a thesis submitted by the first author to the University of the Philippines Los Baños, in partial fulfillment of the requirements for the degree of Master of Science.	en_US
dc.description.abstract	The prokaryotic diversity associated with serpentinization-driven Manleluag Hyperalkaline (pH 11) Spring in Pangasinan, Philippines was investigated. DNA extracted directly from the sediment samples was used to construct clone libraries based on bacterial and archaeal 16S rRNA gene sequences. Phylogenetic analysis of 16S rRNA gene sequences from the clone library revealed that the clones were grouped into Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Cyanobacteria, Bacteroidetes, and Firmicutes. Analysis of the archaeal 16S rRNA clones revealed the presence of sequences associated with members of Euryarchaeota and Thaumarchaeota. Most of the sequences from Euryarchaeota were related to Methanobacteria and Methanomicrobia. Some clones show little affiliation with known taxa and may represent novel sequences of organisms adapted to the hyperalkaline conditions. The populations found suggest the type of metabolisms that drive this specific environment, which include ammonia oxidation, and hydrogen-based and methanogenic metabolisms. This study represents the first analysis of prokaryotic diversity from community DNA of a hyperalkaline environment in the Philippines.	en_US
dc.description.sponsorship	The Commission on Higher Education is greatly acknowledged for funding support through the Commission on Higher Education Science and Engineering Graduate Scholarship (CHED-SEGS) Program. Likewise, we would like to acknowledge Dr. Teofilo A. Abrajano Jr. for providing us with additional laboratory supplies.	en_US
dc.language.iso	en	en_US
dc.subject	16S rRNA gene sequence	en_US
dc.subject	alkaliphiles	en_US
dc.subject	hyperalkaline spring	en_US
dc.subject	phylogenetic analysis	en_US
dc.subject	serpentinization	en_US
dc.title	Prokaryotic Community Analysis of a Hyperalkaline Spring in the Philippines Using 16S rRNA Gene Clone Library Construction	en_US
dc.type	Article	en_US